|
KMID : 0644319990050010103
|
|
Journal of Korean Oriental Oncology
1999 Volume.5 No. 1 p.103 ~ p.118
|
|
|
Effects of Trichosanthes kirilowii Maxim and Scutellaria baicalensis Georgi on IL-1 β-induced GnT-3 and V in Melanoma B-16
|
|
|
|
|
Abstract
|
|
|
The activity of N-acetylglucosamitnyltransferase(GnT) Ⅲ and V on a Melanoma B-16 was examined
after incubation with interleukin 1 (IL-l ). While augumatting cell proliferation, IL-1 resulted in a
decrease of GnT-Ⅲ activity and an increase of GnT-V activities. Consist-u with this, Mdamnu B-16
cultured with IL-l showcd increased affinlity to Daturam stramonium latin, which raogfuzes asialo-tri- and asialeo-tetra-antenery N-linked oligosaccharides. These results indicate that IL-1 modulate glycosyloaderse activity and the oligosaccharide sVtuture of target cells.
On the omer hand, to investigate whether or not TKM-SG affect GnT-V gene expression in lung
metastatic carcinoma, we used RT-PCR methods. TKM-SG treated cell lines showed low leve1s of
secretion of GnT-V mRNA transcription·as elucidated by RT-PCR. Thus, with together lower GnT-V
acnuty levels in the medium, TKM-SG was highly enative for lung cancer metastasis treatment and it
was concluded that the medicine can be used as a potent anti-lung cancer metastasis medidcine.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|